Sangamithirai Subramanian Parimalam

Domaines d'expertises et de recherche

Biotechnologie. Ingénierie et Biofabrication de protéines recombinantes. Technologies Microfluidiques à cellule unique et ARN. Biomatériaux.

Activités de recherche

• Développement de biomolécules et de systèmes cellulaires de nouvelle génération
• Développement de technologies microfluidiques à cellule unique
• Développement de bioadhésifs

Publications

1. Qin,D., Gibbons, A., Ito, M., Subramanian Parimalam, S., Jiang, H., Karahan, H.E., Ghalei, B., Yamaguchi, D., Tanaka, K., Namasivayam, G.P., Sivaniah, E. (2022). “Structural colour enhanced microfluidics” Nat Commun, 13, 2281, doi: 10.1038/s41467-022-29956-4. (IF: 14.7)

2. Ramasamy, S., Mishra, S., Sharma, S., Subramanian Parimalam, S., Thangavel, V., Fujita, Y., Kovi, B., Sugiyama, H, Namasivayam, G.P., (2022) “An informatics approach to distinguish RNA modifications in nanopore direct RNA sequencing”, Genomics, 114, 110372, doi:10.1016/j.ygeno.2022.110372.   (IF: 3.4)

3. Subramanian Parimalam, S., Abdelmoez, M., Tsuchida, A., Sotta, N., Tanaka, M., Kuromori, T., Fujiwara, T., Hirai, M., Yokokawa, R., Oguchi, Y., & Shintaku, H. (2021) “Targeted permeabilization of cell wall and extraction of charged molecules from single cells in intact plant clusters using focused electric field”. Analyst, doi: 10.1039/D0AN02163F. (IF: 3.6)

4. Subramanian Parimalam, S., Oguchi, Y., Abdelmoez, M., Tsuchida, A., Ozaki, Y., Yokokawa, R., & Shintaku, H. (2018). “Electrical lysis and RNA extraction from single cells fixed by dithiobis(succinimidyl propionate)”. Anal Chem, 90, 12512. doi:10.1021/acs.analchem.8b02338.     (IF: 6.7)

Conference proceedings

1. Subramanian Parimalam, S., Sotta, N., Kuromori, T., Fujiwara, T., Yokoto Hirai, M., & Shintaku, H. “Extraction of RNA from an intact single plant cell with cell wall using focused electric field” in Proceedings of IEEE International Symposium on Micro-NanoMechatronics and Human Science, Nagoya University, Japan, 1-4 December (2019), 275. DOI:10.1109/MHS48134.2019.9249352

2. Subramanian Parimalam, S., Sotta, N., Fujiwara, T., Yokoto Hirai, M., & Shintaku, H. “Selective and direct extraction of cytoplasmic RNA from single plant cell in an intact tissue via focused electric field” in Proceedings of EMBO workshop on Single Cell Biology, Tokyo, 20-22 May (2019).

3. Subramanian Parimalam, S., Oguchi, Y., Abdelmoez, M., Tsuchida, A., Ozaki, Y., Yokokawa, R., Kotera, H., & Shintaku, H. “Electrical cell lysis and isotachophoresis aided RNA extraction from fixed single cells” The All-RIKEN Workshop, Wakoshi, 14 December (2018).

 4. Subramanian Parimalam, S., Oguchi, Y., Abdelmoez, M., Tsuchida, A., Ozaki, Y., Yokokawa, R., Kotera, H., & Shintaku, H. “Isotachophoresis-based RNA extraction from fixed single cells” in the Proceedings of the Symposium on Micro-Nano Science and Technology, B Chem., vol. 2018.9, 30am3PN37, 30 October (2018). DOI: https://doi.org/10.1299/jsmemnm.2018.9.30am3PN37

5. Subramanian Parimalam, S., Oguchi, Y., Abdelmoez, M., Tsuchida, A., Ozaki, Y., Yokokawa, R., Kotera, H., & Shintaku, H. “Extraction and fractionation of RNA from fixed single cells” TMN Poster Session, Yamanaka, 28 September (2018).

6. Subramanian Parimalam, S., Kubo, M., Yokokawa, R., Kotera, H., and Shintaku, H. “Differentiating cell-cycle heterogeneity by pico-liter well based microRNA cytometry” TM Network workshop, Yamagata, 03 August (2016).

7. Subramanian Parimalam, S., Yokokawa, R., Kotera, H., & Shintaku, H. “Massively parallel quantification of miRNA in single cells via duplex-specific nuclease reaction in pico-liter wells” 2016 International conference of Microfluidics, Nanofluidics, and Lab-on-a-chip, Dalian, China, 10th June (2016), Track13-292, 287-288.

8. Subramanian Parimalam, S., Kubo, M., Yokokawa, R., Kotera, H., & Shintaku, H. “One step assay for miRNA quantification in single cells via duplex-specific nuclease induced signal amplification” ナノミクス第3回若手研究者交流会, Kyoto University, Katsura, 18 November (2015)