Evan Andre Mercier

Prix et distinctions

• (2012) Alexander Grahm Bell Canada Graduate Scholarship. Natural Sciences and Engineering Research Council of Canada (NSERC). (Prize / Award).
• (2011) Alberta Ingenuity Fund Student Scholarship. Alberta Innovates. (Prize / Award).

Financement

• Grant. (Awarded). Principal Applicant. Conserved structural dynamics of outer membrane channels in Acinetobacter baumannii as potential drug targets. Canadian Institutes of Health Research (CIHR). Bridge grant: Fall 2022. 815 000 $. (2023-2028)
• Grant. (Awarded). Principal Applicant. Multidrug-efflux pumps and their role in antimicrobial resistance. Fonds de recherche du Québec - Santé (FRQS). Research Scholar - Junior 1. 260 000 $. (2023-2027)
• Grant. (Awarded). Principal Applicant. Multiscale structural dynamics of membrane protein complexes. Natural Sciences and Engineering Research Council of Canada (NSERC). Discovery Grant. 195 000 $. (2022-2027)
• Grant. (Awarded). Co-applicant. Expression and mutation of multidrug efflux pumps in Acinetobacter baumannii as drivers of clinically relevant antimicrobial resistance.. Centre de Recherche du Centre Hospitalier de l'Université de Sherbrooke Inc. (CRCHUS) (Sherbrooke, QC). Structuring grants. 80 000 $. (2022-2023)

Publications

Articles de revue

• Mercier, E. Wang, X. Bögeholz, L.A.K.* Wintermeyer, W. Rodnina, M.V. (2022). Cotranslational biogenesis of membrane proteins in bacteria. Frontiers in Molecular Biosciences 9 871121. (Published).
• Bögeholz, L.A.K.*, Mercier, E., Wintermeyer, W., Rodnina, M.V. (2021). Kinetic control of nascent protein biogenesis by peptide deformylase. Scientific Reports 11 24457. (Published).
• Mercier, E., Wang, X., Maiti, M., Wintermeyer, W., Rodnina, M.V. (2021). Lateral gate dynamics of the bacterial translocon during cotranslational membrane protein insertion. Proceedings of the National Academy of Sciences of the United States of America 118 (26), e2100474118. DOI. (Published).
• Mercier, E., Wintermeyer, W., Rodnina, M.V. (2020). Co-translational translocon insertion andtopogenesis of bacterial membrane proteins. The EMBO Journal 29 e104054. (Published).
• Girodat, D.*, Mercier, E., Gzyl, K.E.*, Wieden, H-J. (2019). ElongationFactor Tu's Nucleotide Binding Is Governed by a Thermodynamic Landscape Uniqueamong Bacterial Translation Factors. Journal of the American Chemical Society 141 10236-10246. (Published).
• Zaghouani, M., Boegeholz, L.A.K.*, Mercier, E., Wintermeyer, W., Roche, S.P. (2019). Total synthesis of(±)-fumimycin and analogues for biological evaluation as peptide deformylaseinhibitors. Tetrahedron 75 3216-3230. (Published).
• Mercier, E., Rodnina, M.V. (2018). Co-translational folding trajectory of the HemKHelical Domain. Biochemistry 57 3460-3464. (Published).
• Mercier, E., Holtkamp, W., Rodnina, M.V., Wintermeyer, W. (2017). Signal recognition particle binds to translatingribosomes before emergence of a signal anchor sequence. Nucleic Acids Research 45 11858-11866. (Published).
• Ranjan, A., Mercier, E., Bhatt A.*, Wintermeyer, W. (2017). Signal recognition particle prevents N-terminalprocessing of bacterial membrane proteins. Nature Communications 8 15562. (Published).
• De Laurentiis, E.I., Mercier, E., Wieden, H-J. (2016). Pseudomonas aeruginosaThe C-terminal helix of elongation factor Ts tunes EF-Tu dynamics tomodulate nucleotide exchange. Journal of Biological Chemistry 291 23136-23148. (Published).
• Mercier, E., Girodat, D.*, Wieden, H-J. (2015). A conserved P-loop anchor limits the structuraldynamics that mediate nucleotide dissociation in EF-Tu. Scientific Reports 5 7677. (Published).
• Rosler, K.S.*, Mercier, E., Andrews, I.C., Wieden, H-J. (2015). Histidine 114 is critical for ATP hydrolysis bythe universally conserved ATPase YchF. Journal of Biological Chemistry 290 18650-18661. (Published).
• Jenna Friedt Fern MV Leavens Evan Mercier Hans-Joachim Wieden Ute Kothe. (2014). An arginine/aspartate network in the active site of bacterial TruB is critical for catalyzing pseudouridine formation. Nucleic Acids Research 42 (6), 3857-3870. DOI. (Published).
• Hans-Joachim Wieden Evan Mercier John Gray Brett Steed Davis Yawney. (2010). A combined molecular dynamics and rapid kinetics approach to identify conserved three-dimensional communication networks in elongation factor Tu. Biophysical Journal 99 (11), 3735-3743. (Published).
• Evan Mercier Branden Fonovic Chris Henry Ohyun Kwon Travis Dudding. (2007). Phosphine triggered [3+ 2] allenoate–acrylate annulation: a mechanistic enlightenment. Tetrahedron letters 48 (20), 3617-3620. (Published).
• Travis Dudding Ohyun Kwon Evan Mercier. (2006). Theoretical rationale for regioselection in phosphine-catalyzed allenoate additions to acrylates, imines, and aldehydes. Organic Letters 8 (17), 3643-3646. (Published).

Autres contributions

Cours enseignés

• Introduction to transient kinetic methods. A081. (2017-09-01 à 2022-09-01).Göttingen Graduate Center for Neurosciences, Biophysics, and Molecular Biosciences. Niveau : Graduate.
• Research seminars (1 hour seminar per year). (2017-01-01 à 2022-01-01).Göttingen Graduate Center for Neurosciences, Biophysics, and Molecular Biosciences. Niveau : Graduate.
• Proteins, Enzymes, and Nucleic Acids. BCHM 3100. (2009-09-01 à 2012-12-21).
• Bioenergetics and Metabolism. BCHM 3300. (2009-01-01 à 2009-04-30).
• Organic Chemistry I. CHEM 2500. (2008-09-01 à 2008-12-19).
• Organic Chemistry II. CHEM 2600. (2008-06-02 à 2008-04-22).
• Metabolic Biochemistry. BCHM 3P01. (2006-09-01 à 2006-12-29).

Activités de collaboration internationale

• Researcher. Germany. Collaborative research center on compartmental gates and contact sites in cells (SFB 1190, German Research Foundation). I am a researcher in a project addressing co-translational protein insertion into the bacterial plasma membrane. I work with the other 15 groups within the collaborative research center in knowledge transfer, and technique expertise.

Présentations

• Mercier, E. (2022). Membrane protein dynamics during insertion at the bacterial translocon. Invited seminar: NanoCell graduate program. Linz, Switzerland
• (2022). Real-time dynamics of bacterial membrane proteins during insertion at the translocon. FASEB: Protein Folding in the Cell. Dublin, Ireland
• Mercier, E. (2021). Real-time observation of co-translational membrane protein insertion at the bacterial translocon. Protein synthesis and translational control (International Conference). Heidelberg, Germany
• Mercier, E. (2018). Signal recognition particle binds to translating ribosomes before emergence of a signal anchor sequence. Gordon research conference on protein transport across cell membranes. (International Conference). Galveston, United States of America
• Mercier, E. (2018). Translation by the ribosome coordinates co-translational events in protein biogenesis. University of Tübingen: Invited seminar (Local). Tübingen, Germany
• Mercier, E. (2017). The signal recognition particle prevents N-terminal processing of nascent bacterial membrane proteins. EMBO conference on protein transocation and cellular homeostasis. (International Conference). Dubrovnik, Croatia