Daniel Lafontaine

Prix et distinctions

• (2011) FRQS Scholar Junior II, Salary Award. Fonds de recherche du Québec - Santé (FRQS). (Prix / Récompense).
• (2011) CIHR New Investigator Award. Instituts de recherche en santé du Canada. (Prix / Récompense).
• (1998) EMBO Practical Course (Univ. Copenhagen). EMBO. (Distinction).
• "Prix Relève" given by the Fondation du CHUS/Royal Bank of Canada given to the most promising young researcher. Centre Hospitalier Univ. de Sherbrooke. (Prix / Récompense).
• Accelerated promotion to the Professor rank (attributed only to outstanding candidates). Université de Sherbrooke. (Distinction).
• Alumnus of the Year 2010 (Riboclub organization). Ribo-Club. (Distinction).
• Best Research Discovery in Quebec in 2010 according to a public vote. Québec Science. (Distinction).
• FRQS Scholar Senior. Fonds de recherche du Québec - Santé (FRQS). (Prix / Récompense).
• Mérite Estrien (Attributed to people having a high research impact in society). La Tribune / Radio-Canada. (Distinction).
• Part of the Top 10 Research Discoveries in 2010 according to Québec Science. Québec Sciences. (Distinction).
• Prix Roche (Best scientific achievements during PhD studies). Roche Diagnostic. (Distinction).

Financement

• Subvention. (En cours d’évaluation). Demandeur principal. Study of riboswitch regulatory mechanisms in Escherichia coli. Conseil de Recherches en Sciences Naturelles et Génie du Canada (CRSNG). 36 000 $. (2020-2025)
• Subvention. (Obtenu). Demandeur principal. Real-time imaging and co-transcriptional probing of riboswitch regulatory mechanisms. Instituts de Recherche en Santé du Canada (IRSC). Operating grant. 699 975 $. (2017-2022)
• Subvention. (Obtenu). Collaborateur. Exploration of the TPP riboswitch as a new target for antibiotics. JPIAMR. Joint Programming Initiative on Antimicrobial Resistance. 1 782 500 $. (2019-2021)
• Subvention. (Obtenu). Chercheur principal. Characterization of transcriptional pausing data. Université de Sherbrooke, Faculté des sciences. Aide à la Recherche: Soutien facultaire au recrutement aux études supérieures. 18 000 $. (2019-2020)
• Subvention. (Obtenu). Demandeur principal. Importance of transcriptional pausing in bacterial riboswitch regulation. Conseil de Recherches en Sciences Naturelles et Génie du Canada (CRSNG). 36 000 $. (2019-2020)
• Subvention. (Obtenu). Chercheur principal. Deciphering TPP recognition by a bacterial riboswitch in real-time. Université de Sherbrooke, Faculté des sciences. Aide à la Recherche: Soutien facultaire au recrutement aux études supérieures. 66 000 $. (2017-2019)
• Subvention. (Obtenu). Chercheur principal. The role of metabolite binding on transcriptional pausing in E. coli riboswitches. Université de Sherbrooke, Faculté des sciences. Aide à la Recherche: Soutien facultaire au recrutement aux études supérieures. 66 000 $. (2017-2019)
• Subvention. (Obtenu). Chercheur principal. Studying the role of transcription factors on transcriptionnal pauses. Université de Sherbrooke, Faculté des sciences. Aide à la Recherche: Soutien facultaire au recrutement aux études supérieures. 22 000 $. (2018-2018)

Publications

Articles de revue

• Chauvier A*, Nadon JF*, Grondin JP*, Lamontagne AM*, Lafontaine DA. (2019). Role of a hairpin-stabilized pause in the Escherichia coli thiC riboswitch function. RNA Biology 16 (8), 1066-1073. (Article publié).
• McCluskey K, Boudreault J*, St-Pierre P*, Perez-Gonzalez C, Chauvier A*, Rizzi A, Beauregard PB, Lafontaine DA, Penedo JC. (2019). Unprecedented tunability of riboswitch structure and regulatory function by sub-millimolar variations in physiological Mg2. Nucleic Acids Res 47 (12), 6478-6487. (Article publié).
• Bastet L*, Turcotte P*, Wade JT, Lafontaine DA. (2018). Maestro of regulation: Riboswitches orchestrate gene expression at the levels of translation, transcription and mRNA decay. RNA Biol 15 679-682. (Article publié).
• Yan LH, Le Roux A, Boyapelly K, Lamontagne AM*, Archambault MA*, Picard-Jean F*, Lalonde-Seguin D, St-Pierre E*, Najmanovich RJ, Fortier LC, Lafontaine DA, Marsault E. (2018). Purine analogs targeting the guanine riboswitch as potential antibiotics against Clostridioides difficile. Eur J Med Chem 143 755-768. (Article publié).
• Laterreur N, Lemieux B, Neumann H, Berger-Dancause JC*, Lafontaine D, Wellinger RJ. (2018). The yeast telomerase module for telomere recruitment requires a specific RNA architecture. RNA 24 1067-1079. (Article publié).
• Chauvier A*, Picard-Jean F*, Berger-Dancause JC*, Bastet L*, Naghdi MR, Dube A*, Turcotte P*, Perreault J, Lafontaine DA. (2017). Nature Communications 8 13892. (Article publié).
• Dussault AM*, Dubé A*, Jacques F*, Grondin JP*, Lafontaine DA. (2017). Ligand Recognition and Helical Stacking Formation are Intimately Linked in the SAM-I Riboswitch Regulatory Mechanism. Nucleic Acids Res 23 1539-1551. (Article publié).
• Bastet L*, Chauvier A*, Singh N, Lussier A*, Lamontagne AM, Prevost K, Masse E, Wade JT, Lafontaine DA. (2017). Translational control and Rho-dependent transcription termination are intimately linked in riboswitch regulation. Nucleic Acids Res 45 7474-7486. (Article publié).
• Perez-Gonzalez C, Lafontaine DA°, Penedo JC° (°corresponding, review article). (2016). Fluorescence-Based Strategies to Investigate the Structure and Dynamics of Aptamer-Ligand Complexes. Frontiers in chemistry 4 33-55. (Article publié).
• Comeau MA*, Lafontaine DA°, Abou Elela S° (°corresponding). (2016). The catalytic efficiency of yeast ribonuclease III depends on substrate specific product release rate. Nucleic Acids Res 44 7911-7921. (Article publié).
• Lussier A*, Bastet L*, Chauvier A*, Lafontaine DA. (2015). A kissing-loop is important for BtuB riboswitch ligand sensing and regulatory control. J Biol Chem 290 26739-26751. (Article publié).
• Bordeleau E, Purcell EB, Lafontaine DA, Fortier LC, Tamayo R, Burrus V. (2015). Cyclic di-GMP riboswitch-regulated type IV pili contribute to aggregation of Clostridium difficile. J Bacteriol 197 819-832. (Article publié).
• Smith-Peter E*, Lamontagne AM, Lafontaine DA. (2015). Role of lysine binding residues in the global folding of the lysC riboswitch. RNA Biol 12 1372-1382. (Article publié).
• St-Pierre P*, McCluskey K, Shaw E, Penedo JC°, Lafontaine DA° (°corresponding, review article). (2014). Fluorescence tools to investigate riboswitch structural dynamics. Biochim Biophys Acta 1839 1005-1019. (Article publié).
• Laterreur N, Eschbach S*, Lafontaine DA, Wellinger R. (2013). A new telomerase RNA element that is critical for telomere elongation. Nucleic Acids Res 41 7713-24. (Article publié).
• Ster C, Allard M, Boulanger S, Lamontagne Boulet M, Mulhbacher J*, Lafontaine DA, Marsault E, Lacasse P, Malouin M. (2013). Experimental treatment of Staphylococcus aureus bovine intramammary infection using a guanine riboswitch ligand analog. Journal of Dairy Science 96 1000-8. (Article publié).
• Chinnappan R*, Dubé A*, Lemay JF*, Lafontaine DA. (2013). Fluorescence monitoring of riboswitch transcription regulation using a dual molecular beacon assay. Nucleic Acids Res 41 e106. (Article publié).
• Lalaouna D, Simoneau-Roy M*, Lafontaine DA, Massé E (review article). (2013). Regulatory RNAs and target mRNA decay in prokaryotes. BBA - Gene Regulatory Mechanisms 1829 742-7. (Article publié).
• Dalgarno P, Bordello J, Morris R, St-Pierre P*, Dubé A*, Samuel L*, Lafontaine DA°, Penedo JC°(°corresponding) "Top 5% of papers in terms of originality, significance and scientific excellence". (2013). Single-molecule denaturation of riboswitches. Nucleic Acids Res 41 4253-65. (Article publié).
• Caron MP*, Bastet L*, Lussier A*, Simoneau-Roy M*, Massé E°, Lafontaine DA° (°corresponding). "Selected by Faculty of 1000 and was highlighted in Nature Reviews Microbiology". (2012). Dual-acting riboswitch control of translation initiation and mRNA decay. Proc Natl Acad Sci USA 109 e3444-53. (Article publié).
• Eschbach SH*, St-Pierre P*, Penedo JC°, Lafontaine DA° (°corresponding, review article). (2012). Folding of the SAM-I riboswitch: A tale with a twist. RNA Biology 9 535-41. (Article publié).
• Lemay JF*, Desnoyers G, Blouin S*, Heppell B*, Bastet L*, St-Pierre P*, Massé E°, Lafontaine DA° (°corresponding). (2011). Comparative study between transcriptionally- and translationally-acting adenine riboswitches reveals key differences in riboswitch regulatory mechanisms. PLoS Genetics 7 e1001278. (Article publié).
• Tremblay R*, Lemay JF*, Blouin S*, Mulhbacher J*, Bonneau E, Legault P, Dupont P, Penedo JC, Lafontaine DA. (2011). Constitutive regulatory activity of an evolutionary-excluded riboswitch variant. The Journal of Biological Chemistry 286 27406-15. (Article publié).
• Blouin S*, Chinnappan R*, Lafontaine DA. (2011). Folding of the lysine riboswitch: importance of peripheral elements for transcriptional regulation. Nucleic Acids Res 39 3373-81. (Article publié).
• Heppell B*, Blouin S*, Dussault AM*, Mulhbacher J*, Ennifar E, Penedo JC°, Lafontaine DA°. (°corresponding). "Selected for the news and views of the same journal and was selected by Falculty of 1000.". (2011). Molecular insights into the ligand-controlled organization of the SAM-I riboswitch. Nature Chemical Biology 7 384-868. (Article publié).
• Bastet L*, Dubé A*, Massé E, Lafontaine DA (review article). (2011). New insights into riboswitch regulation mechanisms. Molecular Microbiology 80 1148-54. (Article publié).
• Mulhbacher J*, Brouillette E, Allard M, Fortier LC, Malouin F°, Lafontaine DA° (°corresponding). "Mentionned in Research highlighs of Nature Chemical Biology". (2010). Novel riboswitch ligand analogs as selective inhibitors of guanine-related metabolic pathways. PLoS Pathogens 6 (4), e1000865-e1000865. (Article publié).
• Delfosse V, Bouchard P, Bonneau E, Dagenais P, Lemay JF*, Lafontaine DA°, Legault P° (°corresponding) "Selected by Faculty of 1000". (2010). Riboswitch structure: an internal residue mimicking the purine ligand. Nucleic Acids Res 38 32057-68. (Article publié).
• Caron MP*, Lafontaine DA, Massé E (review article) "Selected as the Most popular issue of the month". (2010). Small RNA-mediated regulation at the level of transcript stability. RNA Biol 7 140-4. (Article publié).
• Mulhbacher J*, St-Pierre P*, Lafontaine DA (review article). (2010). Therapeutic applications of ribozymes and riboswitches. Current Opinion in Pharmacology 10 (5), 551-556. (Article publié).
• Blouin S*, Mulhbacher J*, Penedo JC°, Lafontaine DA° (°corresponding, review article). (2009). Riboswitches: Ancient and Promising Genetic Regulators. ChemBioChem 10 400-16. (Article publié).
• Heppell B*, Lafontaine DA. (2008). Folding of the SAM Aptamer is Determined by the Formation of a K-turn-dependent Pseudoknot. Biochemistry 47 1490-9. (Article publié).
• Blouin S*, Lafontaine DA. (2007). A loop loop interaction and a K-turn motif located in the lysine aptamer domain are important for the riboswitch gene regulation control. RNA 13 (8), 1256-67. (Article publié).
• Lemay JF*, Lafontaine DA. (2007). Core requirements of the adenine riboswitch aptamer for ligand binding. RNA 13 (3), 339-50. (Article publié).
• Mulhbacher J* , Lafontaine DA. (2007). Ligand recognition determinants of guanine riboswitches. Nucleic acids research 35 (16), 5568-80. (Article publié).
• Lemay JF*, Penedo JC, Tremblay R*, Lilley DM, Lafontaine DA. (2006). Folding of the adenine riboswitch. Chemistry & biology 13 (8), 857-68. (Article publié).

Livres

• Singh N, Lange M J, Scarborough R J, Jiang X, Carter J R, D’Anjou F, Lee S-W, Bajaj P, Korbi A, Daldrop P, Colizzi F, Tripp V T, Pedrolli D B, Fowler C C, Lünse C E, Baird N J, Soulière M F, Yan A, Thomas J M et al. (2014). Therapeutic Applications of Ribozymes and Riboswitches - Methods in Molecular Biology. (1103). États-Unis : Humana Press. (Article publié).

Chapitres de livre

• Perez-Gonzalez C, Grondin JP*, Lafontaine DA, Carlos Penedo J. (2016). Biophysical Approaches to Bacterial Gene Regulation by Riboswitches. Leake M C. Biophysics of Infection (157-191). États-Unis : Springer International Publishing. (Article publié).
• Blouin S*, Craggs TD, Lafontaine DA, Penedo JC. (2015). Functional studies of DNA-protein interactions using FRET techniques. Leblanc B P, Rodrigue S. Methods Mol Biol (115-141). États-Unis : Humana Press. (Article publié).
• Chauvier A*, Lafontaine DA. (2015). Probing of nascent riboswitch transcripts. Leblanc B P, Rodrigue S. Methods Mol Biol (109-114). États-Unis : Humana Press. (Article publié).
• Colizzi F, Lamontagne AM*, Lafontaine DA, Bussi G. (2015). Probing riboswitch binding sites with molecular docking, focused libraries, and in-line probing assays. Lafontaine D A, Dubé A. Methods Mol Biol (141-151). États-Unis : Humana Press. (Article publié).
• Boudreault J*, Perez-Gonzalez DC, Penedo JC, Lafontaine DA. (2015). Single-molecule approaches for the characterization of riboswitch folding mechanisms. Leblanc B P, Rodrigue S. Methods Mol Biol (101-107). États-Unis : Humana Press. (Article publié).
• Eschbach SH*, Lafontaine DA. (2014). RNA conformational changes analyzed by comparative gel electrophoresis. Waldsich C. Methods Mol Biol (255-265). États-Unis : Humana Press. (Article publié).
• McCluskey K, Shaw E, Lafontaine DA, Penedo JC. (2014). Single-molecule fluorescence of nucleic acids. Engelborghs Y, Visser A J W G. Methods Mol Biol (759-791). États-Unis : Humana Press. (Article publié).
• Shaw E, St-Pierre P*, McCluskey K, Lafontaine DA, Penedo JC. (2014). Using sm-FRET and denaturants to reveal folding landscapes. Pecoraro V L. Methods Enzymol (313-341). Pays-Bas : Elsevier. (Article publié).
• Heppell B*, Mulhbacher J*, Penedo JC, Lafontaine DA. (2009). Application of Fluorescence Measurements for Characterization of Riboswitch-Ligand Interactions. Serganov A. Methods Mol Biol (25-37). États-Unis : Humana Press. (Article publié).
• Blouin S*, Craggs T, Lafontaine DA, Penedo JC. (2009). Functional studies of DNA-protein interactions using FRET techniques. Leblanc B, Moss T. Methods Mol Biol (475-502). États-Unis : Humana Press. (Article publié).
• Lemay JF*, Mulhbacher J*, Penedo JC, Lafontaine DA. (2009). Molecular Basis of RNA-Mediated Gene Regulation on the Adenine Riboswitch by Single-Molecule Approaches. Serganov A. Methods Mol Biol (65-76). États-Unis : Humana Press. (Article publié).

Documents de travail

• St-Pierre P, Grondin J and Lafontaine DA. (2016). Folding of the adenine riboswitch core domain monitored at the singlemolecule level.
• St-Pierre P, Jacques S, Picard-Jean F, Penedo J C and Lafontaine DA. (2016). Folding pathway of the add adenine riboswitch monitored using singlemolecule FRET.
• Nadon JF, Lafontaine DA. (2016). Influence of NPOM-dT photocaged DNA on transcription by E. coli and T7 RNA polymerases.
• Dussault AM*, Dubé A, Jacques F and Lafontaine DA. (2016). Ligand Recognition and Helical Stacking Formation are Intimately Linked in the SAM-I Riboswitch Regulatory Mechanism.
• Bastet L, Chauvier A, Singh N, Lussier A, Wade J and Lafontaine DA. (2016). Riboswitch-mediated translation repression directs Rho termination and mRNA decay through a single RNA element.
• Boudreault J, Perez-Gonzalez DC, Heppell B, Penedo C and Lafontaine DA. (2016). SAM-I riboswitch mutagenesis reveals clues on a unique ligand dependent stem rotation mechanism at single-molecule level.
• Bastet L, Lamontagne AM, Massé E and Lafontaine DA. (2016). Small RNA and riboswitch btuB translation inhibition modulate mRNA levels through different mechanisms.

Articles de conférence

• Hien E*, Michaud A*, Vanutelli A, Vezina-Bedard AS*, Yague C. (2019). L'ARN, bien plus qu'un messager. JVSS 2019 ****Best poster presentation and prize from the public****. (Article accepté).
• Lamontagne AM*, St-Pierre P*, Lafontaine DA. (2019). RNA polymerase walking on photocleavable solid phase for sm-FRET analysis of RNA folding. Riboclub 2019. (Article accepté).
• Grondin JP*,Chauvier A*, St-Pierre P*, Nadon JF*, Turcotte P*, Geffroy M*, Perez-Gonzalez C, Eschbach SH*, Simoneau-Roy M*, Lamontagne AM*, Massé E, Penedo JC, Lafontaine DA. (2019). Real time imaging of co-transcriptional folding and translation regulation. RNA 2019. (Article accepté).
• Nadon JF*, Epshtein V, Nudler E, Lafontaine DA. (2019). Shedding light on transcriptional pausing: A new photolabile roadblock allows more reliable studies of elongation dynamics. Riboclub 2019. (Article accepté).
• Chauvier A*, St-Pierre P*, Nadon JF*, Geffroy M*, Perez-Gonzalez C, Eschbach SH*, Simoneau-Roy M*, Lamontagne AM*, Masse E, Penedo JC, Lafontaine DA. (2019). Single-molecule FRET imaging of riboswitch transcriptional complexes. RNA 2019. (Article accepté).
• Hien E*, Chauvier A*, Nadon JF*, Lafontaine DA. (2019). Study of thiC riboswitch transcriptional pauses in Escherichia coli. Canadian Society of Microbiology 2019. (Article accepté).
• Hien E*, Chauvier A*, Nadon JF*, Lafontaine DA. (2019). Study of thiC riboswitch transcriptional pauses in Escherichia coli. Riboclub 2019. (Article accepté).
• Vézina Bédard AS*, Geffroy M*, Martin F, Singh N, Massé É, Wade J, Guillier M, Lafontaine DA. (2019). corA : a new riboswitch in Escherichia coli. RNA 2019. (Article accepté).
• Vézina Bédard AS*, Geffroy M*, Martin F, Singh N, Massé É, Wade J, Guillier M, Lafontaine DA. (2019). corA : a new riboswitch in Escherichia coli. Riboclub 2019. (Article accepté).
• Nadon JF*, Lafontaine DA. (2018). Development of a light-dependent transcriptional roadblock. Riboclub 2018. (Article accepté).
• Grondin J*, Lafontaine DA. (2018). Monitoring the 30S binding dynamics to a tbpA riboswitch in real time. Riboclub 2018. (Article accepté).
• Grondin J*, St-Pierre P, Lamontagne AM, Lafontaine DA. (2018). Monitoring the 30S binding dynamics to a tbpA riboswitch in real time. RNA 2018. (Article accepté).
• Berger-Dancause JF*, Hussein S, Lafontaine DA. (2018). Structural study of the TUNAR RNA. Riboclub 2018. (Article accepté).
• Michaud A*, Lafontaine DA. (2018). Study of thiM mRNA levels in E coli using real-time fluorescence assays. Riboclub 2018. (Article accepté).
• Vezina-Bedard AS*, Geffroy M*, Guillier M, Lafontaine DA. (2018). corA : A new riboswitch in Escherichia coli?. Riboclub 2018. (Article accepté).
• Turcotte P*, Chauvier A*, Bastet L*, Singh N, Wade JT, Lafontaine DA. (2017). A genetic regulation hotspot in the lysC riboswitch. Riboclub 2017. (Article publié).
• Michaud A*, Chauvier A*, Lafontaine DA, Fortier LC. (2017). The glycine riboswitches in Clostridium difficile: a path to explore. Riboclub 2017. (Article accepté).
• Nadon JF*, Lafontaine DA. (2017). Transcriptional Traffic Lights: Using photochemistry to control RNA elongation. Riboclub 2017. (Article accepté).
• Geffroy M*, Stringer AM, Prevost K, Masse E, Lafontaine DA. (2017). mgtAThe riboswitch uses leader peptidetranslation to regulate gene expression in. Riboclub 2017. (Article publié).
• Grondin JP*, Lafontaine DA. (2017). tbpAMonitoringthe 30S binding dynamics to a riboswitchin real time. Riboclub 2017. (Article publié).
• Grondin JP*, St-Pierre P*, Lafontaine D. (2016). Deciphering TPP recognition by a bacterial riboswitch in real-time. Riboclub 2016. (Article publié).
• Chauvier A*, Picard-Jean F, Berger-Dancause JC*, Dubé A*, Naghbi R, Perreault J, Lafontaine D. (2016). Folding and regulation mechanism of the thiC riboswitch. RNA meeting 2016. (Article publié).
• Turcotte P*, Chauvier A*, Bastet L*, Singh N, Caron MP*, Wade JT, Lafontaine DA. (2016). It takes two to tango : When two transcritptionnal mechanisms meet. Riboclub 2016. (Article publié).
• Chauvier A*, St-Pierre P*, Nadon JF*, Perez Gonzalez D-C, Penedo-Esteiro J-C, Lafontaine D. (2016). RNA Folding Dynamics in Transcription Elongation Complexes Monitored at the Single-Molecule Level. Riboclub 2016. (Article publié).
• Nadon JF*, Gonzalez DC, St-Pierre P*, Chauvier A*, Penedo JC, Lafontaine DA. (2016). The Need for Speed. 5th Zing Nucleic Acids Conference 2016 ****Best poster award****. (Article publié).
• Nadon J*, Pérez-González C, St-Pierre P*, Chauvier A*, Penedo JC, Lafontaine DA. (2016). The need for speed: regulation of the thiC riboswitch using PIFE assays. Riboclub 2016. (Article publié).
• Geffroy M*, Masse E, Lafontaine DA. (2016). Translation of a leader peptide is required for mgtA expression in Escherichia coli. Riboclub 2016 ***4th place poster competition, 2nd place voted by the public***. (Article publié).
• Chauvier A*, Picard-Jean F, Dubé A*, Berger-Dancause JC*, Naghbi R, Perreault J, Lafontaine DA. (2015). Folding and regulation mechanism of the thiC riboswitch. Regulating with RNA in Bacteria and Archaea ***2nd place poster competition***. (Article publié).
• Chauvier A*, Picard-Jean F, Dubé A*, Berger-Dancause JC*, Naghbi R, Perreault J, Lafontaine DA. (2015). Folding and regulation mechanism of the thiC riboswitch. RiboClub 2015 ***2nd place poster competition***. (Article publié).
• Boudreault J*, Pérez González DC, Penedo JC, Lafontaine DA. (2015). Folding of the SAM-I riboswitch: A tale with a twist. RiboClub 2015. (Article publié).
• Hérault E*, Massé E, Lafontaine DA. (2015). Regulation by Riboswitches, more than just a “cis history”. RiboClub 2015. (Article publié).
• Geffroy M*, Singh N, Wade J, Massé E, Lafontaine DA. (2015). The mgtA riboswitch in Escherichia coli. Regulating with RNA in Bacteria and Archaea. (Article publié).
• Geffroy M*, Singh N, Wade J, Massé E, Lafontaine DA. (2015). The mgtA riboswitch in Escherichia coli. RiboClub 2015. (Article publié).
• Michaud A*, Smith-Peter E*, Lafontaine DA, Fortier LC. (2015). The riboswitch-controlled GMP synthase GuaA is important for survival and virulence of Clostridium difficile in vitro and in a mouse model of infection. RiboClub 2015. (Article publié).
• Boudreault J*, Heppell B*, Dussault AM*, Lafontaine DA. (2014). Characterization of the folding pathway of the SAM-I riboswitch. Riboclub 2014. (Article publié).
• Chauvier A*, Dubé A*, Naghbi R, Perreault J, Lafontaine DA. (2014). Folding and regulation mechanism of the thiC riboswitch. Riboclub 2014. (Article publié).
• Chauvier A*, Lamontagne AM, Naghbi R, Perreault J, Lafontaine DA. (2014). Folding and regulation mechanism of the thiC riboswitch. RNA 2014. (Article publié).
• Geffroy M*, Lafontaine DA. (2014). Nouveau mécanisme de régulation impliquant le contrôle d’un riborégulateur par la traduction d’un peptide leader. Conférences étudiantes de l'axe Inflammation et Douleur. (Article publié).
• Bastet L*, Lamontagne AM, Massé E, Lafontaine DA. (2014). Riboswitch and small RNA-induced translation inhibition lead to mRNA degradation using different mechanisms. RNA 2014. (Article publié).
• Geffroy M*, Lafontaine DA. (2014). The regulation mechanism of the E. coli mgtA riboswitch. Riboclub 2014. (Article publié).
• Lussier A*, Bastet L*, Lafontaine DA. (2014). What do you need to switch with your B12 riboswitch?. Riboclub 2014. (Article publié).
• Lamontagne AM, Archambault MA, Le-Roux A, Yan LH, Boyapelly K, Lalonde-Seguin D, Marseault E, Fortier LC, Lafontaine DA. (2013). Clostridium difficileNew antibiotic class targeting the guanine riboswitch in. Riboclub 2013. (Article publié).
• Boudreault J*, Heppell B*, Dussault AM*, Eschbach SH*, Lafontaine DA. (2013). Identification of essential nucleotides in the SAM-I riboswitch folding pathway. Riboclub 2013. (Article publié).
• Bastet L*, Caron MP*, Lussier A*, Simoneau-Roy M*, Massé E., Lafontaine DA. (2013). Riboswitch control of translation and mRNA decay. Regulating with RNA in bacteria. (Article publié).
• Lussier A*, Chauvier A*, Lafontaine DA. (2013). Study of translational control of Escherichia coli riboswitches. Riboclub 2013. (Article publié).
• Geffroy M*, Lafontaine DA. (2013). The regulation mechanism of the E. coli mgtA riboswitch. Riboclub 2013. (Article publié).
• Geffroy M*, Bastet L*, Lafontaine DA. (2013). The translationally-acting E. coli AdoCbl riboswitch triggers mRNA decay through translation inhibition. Regulating with RNA in bacteria. (Article publié).
• Bastet L*, Caron MP*, Massé E, Lafontaine DA. (2013). When the small RNA OmrB meet the CoB12 riboswitch in Escherichia coli. RiboClub 2013 ***2nd place poster prize***. (Article publié).
• Chauvier A*, Dubé A*, Lafontaine D. (2013). thiC Deciphering the molecular mechanism of the riboswitch. Riboclub 2013. (Article publié).
• Smith-Peter E*, Lamontagne AM, Lafontaine DA. (2012). Analysis of dynamic ligand capturing and rational design of antibiotics targeting riboswitches. RiboClub 2012. (Article publié).
• Lamontagne AM, Mulhbacher J*, Lafontaine DA. (2012). Characterization of guanine riboswitches for the development of selective analog inhibitors. RNA meeting 2012. (Article publié).
• St-Pierre P*, Penedo JC, Lafontaine DA. (2012). Influence of ligand binding on the loop-loop interaction of the adenine riboswitch aptamer. RNA 2012. (Article publié).
• St-Pierre P*, Penedo JC*, Lafontaine DA. (2012). Influence of the ligand binding on the loop-loop interaction of the adenine binding riboswitch aptamer. RiboClub 2012. (Article publié).
• Comeau MA*, Lafontaine DA, Abou Elela S. (2012). Product-based regulation of RNase III processivity. Riboclub 2012. (Article publié).
• Bastet L*, Lussier A*, Dubé A*, Lafontaine DA. (2012). The butterfly effect of translationally-acting riboswitches. RNA 2012. (Article publié).
• Simoneau-Roy M*, Dubé A*, Caron MP*, Massé E, Lafontaine DA. (2012). The thiB riboswitch of Escherichia coli might be a trans-acting riboswitch. RiboClub 2012. (Article publié).
• Eschbach E*, Dussault AM*, Heppell B*, Lafontaine DA. (2012). Twisting or not twisting the SAM-Iriboswitches. RiboClub 2012. (Article publié).
• Bastet L*, Lussier A*, Dubé A*, Lafontaine DA. (2012). vsDangerous liaisons: The CoB12 riboswitch the small RNA OmrB. Riboclub 2012. (Article publié).
• Smith-Peter E*, Blouin S*, Lafontaine DA. (2011). Caractérisation du site de liaison du riborégulateurlysine. Journée Phare 2011. (Article publié).
• Smith-Peter E*, Blouin S*, Lafontaine DA. (2011). Caractérisation du site de liaison du riborégulateur lysine. ACFAS 2011. (Article publié).
• Eschbach S*, Dussault AM*, Heppell B*, Lafontaine DA. (2011). Comparison between a four-way junctionand a three-way junction SAM-I riboswitch. RiboClub 2011. (Article publié).
• Dubé A*, Simoneau-Roy M*, Caron MP*, Massé E, Lafontaine DA. (2011). Evidences toward different TPP riboswitch regulation mechanisms in Escherichia coli. Regulating with RNA in Bacteria. (Article publié).
• *Comeau MA, Abou Elela S, Lafontaine DA. (2011). Regulation of RNase III mediated RNA metabolism. RiboClub 2011. (Article publié).
• Simoneau-Roy M*, Caron MP*, Massé E, Lafontaine DA. (2011). SroA: une riborégulation agissant en trans ?. ACFAS 2011. (Article publié).
• Bastet *L, Dubé A*, Lafontaine DA. (2011). Studies of the Escherichia coli AdoCbl riboswitch regulation mechanisms. Regulating with RNA in Bacteria. (Article publié).
• Dubé A*, Dussault AM*, Simoneau-Roy S*, Caron MP*, Massé E, Lafontaine DA. (2011). TPP riboswitches exhibit fundamental mechanistic differences in E coli. RiboClub 2011. (Article publié).
• Bastet L*, Dubé A*, Lafontaine DA. (2011). The AdoCbl riboswitch: From the switch to the regulation mechanism. RiboClub 2011. (Article publié).
• Simoneau-Roy M*, Caron MP*, Dubé A*, Massé E, Lafontaine DA ***2nd place for best poster presentation. (2011). The thiB riboswitch of Escherichia coli: A trans-acting riboswitch?. RiboClub 2011. (Article publié).
• Blouin S*, Chinnappan R*, Penedo JC, Lafontaine DA. (2010). Folding of the lysine riboswitch:importance of peripheral elements for transcription regulation. RiboClub 2010. (Article publié).
• Bastet L*, Lafontaine DA*. (2010). In vivo characterization of the Escherichia coli translationally-actingAdoCbl riboswitch. RiboClub 2010. (Article publié).
• Dubé A, Caron MP*, Simoneau-Roy M*, Beaulne S*, Massé E, Lafontaine DA. (2010). In vivo regulation of TPP riboswitches in Escherichia coli. RiboClub 2010. (Article publié).
• Caron MP*, Massé E, Lafontaine DA. (2010). In vivo study of lysine riboswitch mechanism in Escherichia coli. RNA 2010. (Article publié).
• Caron MP*, Massé E, Lafontaine DA. (2010). In vivo study of the lysine riboswitch mechanism in Escherichia coli. RiboClub 2010. (Article publié).
• Comeau MA*, Bisaillon M*, Abou Elela S, Lafontaine DA. (2010). Product-determined turnover rate of dsRNA processing. Riboclub 2010. (Article publié).
• St-Pierre P*, Lemay JF*, Penedo JC, Lafontaine DA. (2010). Single molecule folding of a purine bindingRNA aptamer. RIboClub 2010. (Article publié).
• Caron MP*, Desnoyers G*, Massé E, Lafontaine DA. (2009). In vivo study of lysine riboswitch in E. coli. Canadian Society for Microbiology (CSM). (Article publié).
• Delfosse V, Bouchard P, Bonneau E, Dagenais P, Lemay JF*, Lafontaine DA, Legault P. (2009). Purine riboswitch structure: a unique evolutionnary-excluded structural mimicry. Annual GRASP Symposium. (Article publié).

Propriétés intellectuelles

Brevets

• (2015). Guanine riboswitch binding compounds and their use as antibiotics. US 20150150890 A1. Canada. (Délivré).
• (2015). Guanine riboswitch binding compounds and their use as antibiotics. EP 2440533 B1. Canada. (Délivré).
• (2013). Guanine riboswitch binding compounds and their use as antibiotics. EP 2440533 A4. Canada. (Délivré).
• (2012). Guanine riboswitch binding compounds and their use as antibiotics. EP 2440533 A1. Canada. (Délivré).
• (2012). Guanine riboswitch binding compounds and their use as antibiotics. US 20120122819 A1. Canada. (Délivré).
• (2010). Guanine riboswitch binding compounds and their use as antibiotics. WO2010/142027 A1. Canada. (Délivré).
• (2010). Guanine riboswitch binding compounds and their use as antibiotics. CA 2778931 A1. Canada. (Délivré).

Autres contributions

Gestion d'évènements

• Co-organizer. (2019) Riboclub 2019. (Conférence).
• Co-organizer. (2018) Riboclub 2018. (Conférence).
• Co-organizer. (2017) Riboclub 2017. (Conférence).
• Co-organizer. (2016) Riboclub 2016. (Conférence).
• Co-organizer. (2015) RiboClub 2015. (Conférence).
• Co-organizer. (2014) RiboClub 2014. (Conférence).
• Co-organizer. (2013) Riboclub 2013. (Conférence).
• Co-organizer. (2012) RiboClub 2012. (Conférence).
• Co-organizer. (2011) RiboClub 2011. (Conférence).
• Co-organizer. (2011) Colloque ARN (ACFAS). (Atelier).

Activités de collaboration internationale

• Reasearch advisor. Norvège.
• Research collaborator. États-Unis.
• Research collaborator. Royaume-Uni.

Présentations

• (2019). Real-time imaging of riboswitch transcription elongation. Dundee Nucleic Acids Conference. Dundee, Royaume-Uni
• (2018). Metabolite sensing in the TPP riboswitch is modulated during transcription elongation. BBB and CCBIO seminars. Bergen, Norvège
• (2017). L'importance de prendre une pause (moléculaire) pour bien faire les choses. Institut de Pharmacologie de Sherbrooke. Sherbrooke, Canada
• (2016). Real-time imaging of riboswitch regulatory mechanisms. Zing conference, 5th Nucleic Acid Meeting. Florida, États-Unis
• (2015). Study of riboswitch regulation mechanisms modulating mRNA levels. Regulating with RNA in Bacteria and Archaea Conference (Zing Conference). Mexico, Mexique
• (2015). Study of riboswitch regulation mechanisms modulating mRNA levels. RiboWest 2015. Edmonton, Canada
• (2014). Discontinuous transcription elongation dictates riboswitch sensing and regulation mechanisms. RiboClub 2014. Magog, Canada
• (2014). Dual-acting riboswitch control of translation initiation and mRNA decay. Invited by M Bayfield, Dept of Biology, University of York. Toronto, Canada
• (2014). Dual-acting riboswitch control of translation initiation and mRNA decay. Canadian Society for Chemistry. Vancouver, Canada
• (2014). Le secret du riboswitch. Les midis scientifiques du Cegep de Sherbrooke. Sherbrooke, Canada
• (2013). Dual-acting riboswitch control of translation initiation and mRNA decay. Invited by N Polacek, Dept. of Chemistry and Biochemistry, University of Bern. Bern, Suisse
• (2013). Dual-acting riboswitch control of translation initiation and mRNA decay. Invited by E Ennifar, Dept. Biologie structurale, Universite de Strasbourg. Strasbourg, France
• (2013). Molecular insights into the ligand-controlled organization of SAM-sensing riboswitches. Invited by J Wohnert, Dept. RNA-Strukturbiologie, Goethe-University. Frankfurt, Allemagne
• (2013). Novel riboswitch ligand analogs as selective inhibitors of metabolic pathways. Antibiotic Research Symposium. Bonn, Allemagne
• (2013). Riborégulateurs & applications cliniques. Journée scientifique des étudiants du CRHDQ (Centre de Recherche de l’Hôtel-Dieu de Québec). Québec, Canada
• (2013). Riborégulateurs & applications cliniques. 5ième Colloque de l’Association Collégiale de Biotechnologies. Sherbrooke, Canada
• (2013). Riboswitches & clinical applications. General Microbiology Spring Conference. Manchester, Royaume-Uni
• (2013). Riboswitches as novel antibacterial targets. Invited by biotech companies Néomed et Amorchem. Montréal, Canada
• (2013). Riboswitches as targets for novel classes of antibiotic. Canadian Society of Microbiologists. Ottawa, Canada
• (2013). Riboswitches as targets for novel classes of antibiotic. Canadian Society for Chemistry. Québec, Canada
• (2012). Riborégulateurs & applications cliniques. Symposium TEDx de l'Université de Sherbrooke. Sherbrooke, Canada
• (2012). Riboswitches & clinical applications. International Symposium on Staphylococci and Staphylococcal Infections. Lyon, France
• (2011). Controle du niveau de l'ARN messager du riborégulateur lysine. Biologie et Pharmacologie Structurale (ACFAS). Sherbrooke, Canada
• (2011). Implication du dégradosome dans un nouveau mécanisme de régulation génétique du riboswitch lysine de Escherichia coli. Axe d'infectiologie, Centre de recherches cliniques Université de Sherbrooke. Sherbrooke, Canada
• (2011). In vivo characterization of the lysine riboswitch. Invited by S Zimmerly, Dept. Biological Sciences, University of Calgary. Calgary, Canada
• (2011). Le côté obscur des riborégulateurs: quand un mécanisme de régulation en cache un autre. ACFAS. Sherbrooke, Canada
• (2011). Les mystères du riborégulateur adénosylcobalamine. Axe d'infectiologie, Centre de recherches cliniques Université de Sherbrooke. Sherbrooke, Canada
• (2011). New insights into riboswitch regulatory mechanisms. Eastern Riboclub. Sherbrooke, Canada
• (2011). Novel Riboswitch Ligand Analogs as Selective Inhibitors of Guanine-Related Metabolic Pathways. Technological Advances in RNA Therapy. Montréal, Canada
• (2011). Riborégulateurs & applications cliniques. Journée Phare de l'Institut de Pharmacologie de Sherbrooke. Magog, Canada
• (2011). Riboswitches & clinical applications. Invited by L Ferguson, Boehringer Ingelheim (Québec). Laval, Canada
• (2011). Single-molecule investigations of the folding rates of the pbuE adenine riboswitch. Eastern Riboclub. Orford, Canada
• Bastet L, Dubé A, Lafontaine DA. (2011). The dark side of the B12 riboswitch. Eastern RiboClub. Sherbrooke, Canada
• (2010). Folding and activity of the SAM riboswitch. Alumnus of the Year au RiboClub 2010. Magog, Canada
• (2010). Les riborégulateurs / Riboswitches. Invited at the Institut de Pharmacologie de Sherbrooke. Sherbrooke, Canada
• Comeau MA, Wieden HJ, Kothe U, Bisaillon M, Abou Elela S, Lafontaine DA. (2010). Product- Determined turnover rate of dsRNA processing. 6th annual meeting of the Ribowest. Lethbridge, Canada
• (2010). The regulatory nature of riboswitches determines the ligand binding mechanism. Eastern Riboclub. Orford, Canada
• (2010). Étude in vivo de mécanisme de régulation des riborégulateurs. Axe d'infectiologie, Centre de recherches cliniques Université de Sherbrooke. Sherbrooke, Canada
• Delfosse V, Bouchard P, Bonneau E, Dagenais P, Lemay JF, Lafontaine DA, Legault P. (2009). Etudes structurales du riboswich A adénine de Bacillus subtilis. Groupe Thématique de Biologie. Paris, France
• (2009). Folding and activity of the S-adenosyl methionine riboswitch. Invited by N Polacek, Medical University Division of Genomics and RNomics, Innsbruck Biocenter. Innsbruck, Autriche
• (2009). Folding and activity of the S-adenosyl methionine riboswitch. RNA Ligand Interactions. Frankfurt, Allemagne
• (2009). Folding and activity of the S-adenosyl methionine riboswitch. Invited by C Penedo, Dept. Physics and Astronomy, University of St-Andrews. St-Andrews, Royaume-Uni
• (2009). Folding and activity of the S-adenosyl methionine riboswitch. Invited by D Lilley, Dept. Biochemistry, The University of Dundee. Dundee, Royaume-Uni