Aller au contenu
Trouver un autre spécialiste

Denis Gris

Professeur, Faculté de médecine et des sciences de la santé
FMSS Département de pharmacologie-physiologie

Présentation

Sujet de recherche

Central Nervous System Inflammatory Diseases, Neurodegenerative Diseases, Neuroimmunological Diseases, Spinal Cord Diseases, Neurotoxicity

Disciplines de recherche

Neurosciences, Immunology

Mots-clés

Flow cytometry, Histological techniques, Microglia, astrocytes, Microscopy, Neurodegenaration, Neuroinflammation, Nlrs

Intérêts de recherche

NLRs: A recently discovered family of proteins, Nlrs, are among main regulatory proteins of the immune system. The Nlr family of proteins comprises 23 members in human and 34 in mice. Nlrs can regulate both the innate and adaptive immune system. Upon pathogen recognition, these proteins can activate multiple pro-inflammatory molecular pathways including formation of inflammasome, activation of NFkB and MAP kinase pathways. Nlrs have been implicated in development of autoimmune disorders; however their function in neuroinflammation and neurodegeneration is poorly understood. Right now, the function of Nlrs is an emerging field of study which, so far, has been approached by studying the response of the immune system against pathogens. The main interest of my research is to uncover the role of Nlrs in neuroinflammation. This will open a potential to find novel approaches in regulating the immune response.

Centre de recherche

Centre de recherche du CHUS

Langues parlées et écrites

Anglais, Français, Russe

Diplômes

(2007). (Doctorate, Neuroscience). University of Western Ontario.

(2003). (Diploma, Clinical Flow Cytometry Course). Dartmouth-Hitchcock Medical Center.

(2003). (Master's Thesis, Neuroscience - Masters). University of Western Ontario.

(2001). (Bachelor's, Physiology). University of Western Ontario.

(1996). (Diploma, Doctor of Medicine - M.D.).

Titres de compétence

Introduction to Statistical Learning with Applications in R.

PyImage Search Gurus Course: Python and Open CV in Computer Vision.

The Art of R Programming.

Expérience académique

Associate Professor. (2016-). Université de Sherbrooke. Canada.

Prix et distinctions

  • (2016) Research Scholar Junior 1. Fonds de recherche du Québec - Santé (FRQS). (Prize / Award).
  • (2003) Finalist in Abstract Competition. International Neurotrauma Socitey. (Distinction).
  • (2002) Finalist in Abstract Competition. International Neurotrauma Society. (Distinction).
  • (2001) Graduation with distinction. The University of Western Ontario. (Distinction).
  • (2000) Dean's Honours List. The University of Western Ontario. (Distinction).
  • (2000) Student Opportunity Grant. The University of Western Ontario. (Distinction).

Financement

  • Grant. (Awarded). Principal Applicant. Behavioral signature of Neuroinflamation. Natural Sciences and Engineering Research Council of Canada (NSERC). Dicovery. 120 $. (2019-2024)
  • Grant. (Completed). Principal Applicant. From yeast to mice and humans investigating neurodegenerative diseases. Ministère des Relations Internationales du Québec. Quebec-Bavarian Cooperation. 2 000 $. (2016-2018)
  • Grant. (Awarded). Principal Applicant. Role of neuroinflammation in neurodegeneration. Natural Sciences and Engineering Research Council of Canada (NSERC). Dicovery. 120 $.

Publications

Articles de revue

  • Mahmoud S*, Gharagozloo M, Simard C, Gris D. (2019). Astrocytes Maintain Glutamate Homeostasis in the CNS by Controlling the Balance between Glutamate Uptake and Release. Cells 20 (8), 2-22. (Published).
  • Vilariño-Güell C, Zimprich A, Martinelli-Boneschi F, Herculano B, Wang Z, Matesanz F, Urcelay E, Vandenbroeck K, Leyva L, Gris D, Massaad C, Quandt JA, Traboulsee AL, Encarnacion M, Bernales CQ, Follett J, Yee IM, Criscuoli MG, Deutschländer A, Reinthaler EM, Zrzavy T, Mascia E, Zauli A, Esposito F, Alcina A, Izquierdo G, Espino-Paisán L, Mena J, Antigüedad A, Urbaneja-Romero P, Ortega-Pinazo J, Song W, Sadovnick AD. (2019). Exomesequencing in multiple sclerosis families identifies 12 candidate genes andnominates biological pathways for the genesis of disease. PLOS Genetics 6 (15), 6.
  • Marjan Gharagozloo, Shaimaa Mahmoud, Camille Simard, Kenzo Yamamoto, Diwakar Bobbala, Subburaj Ilangumaran, Albert Lamontagne, Samir Jarjoura, Jean-Bernard Denault, Louis Gendron, Carles Vilariño-Güell, A Dessa Sadovnick, Jenny P. Ting, Abdelaziz Amran2, and Denis Gris. (2019). NLRX1 inhibits the early stages of CNS inflammation and prevents the onset of spontaneous autoimmunity. PlosBiology 16 (17), (Published).
  • Mahmoud S, Gharagozloo M, Simard C, Amrani A, Gris D. (2019). NLRX1Enhances Glutamate Uptake and Inhibits Glutamate Release by Astrocytes. Cells 30 (8), 5. (Published).
  • Katsiaryna V Gris*, Kenzo Yamamoto*, Marjan Gharagozloo*, Shaimaa Mahmoud*, Camille Simard*, Pavel Gris, Denis Gris. (2018). Application of Automated Video Behavioral Assessment: Analysis ofMultiparametric Exhaustive Behavioural Arrays. AIMS 5 (3), 238-251. (Published).
  • Kenzo Yamomomto*, Katya Gris*, Emiliano Fonseca*, Daphnee Houle*, Marjan Gharagozloo*, Denis Gris. (2018). Exhaustive Multi-Parametric Assessment of the Behavioral Array of Daily Activities of Mice Using Supervised Learning Software. Frontiers of Behavioral Neuroscience (Manuscript ID: 348015) 12 1. (Published).
  • Marjan Gharagozloo*, Katsiaryna V. Gris*, Tara Mahvelati*, Abdelaziz Amrani, John R. Lukens and Denis Gris. (2018). NLR-Dependant Regulation of Inflammation in Multiple Sclerosis. Front Immunol 8 2012. (Published).
  • Gharagozloo M*, Mahmoud S*, Simard C*, Mahvelati TM*, Amrani A, Gris D. (2018). Nlrp12The Dual Immunoregulatory function of in T Cell-Mediated Immune Response: Lessons from Experimental Autoimmune Encephalomyelitis. Cells 7 (9), 119-129. (Published).
  • Guindi C, Cloutier A, Gaudreau S, Zerif E, McDonald PP, Tatsiy O, Asselin C, Dupuis G, Gris D, Amrani AA. (2018). Role of the p38 MAPK/C/EBP? Pathway in the Regulation of Phenotype and IL-10 and IL-12 Production by Tolerogenic Bone Marrow-Derived Dendritic Cells. Cells. 7 (12), 33-45. (Published).
  • Thomas Brickler, Armand L. Meza, Sheryl Coutermarsh-Ott, Amanda Hazy, Denis Gris, Michelle H. Theus, Irving C. Allen. (2017). Loss of NLRX1 exacerbates neural tissue damage and NF-kappaB signaling following brain injury. Journal of Immunology 199 (10), 3547-3558. (Published).
  • Erwan Lanchec, Antoine Désilets, François Béliveau, Anthony Flamier, Shaimaa Mahmoud*, Gilbert Bernier, Denis Gris, Richard Leduc and Christine Lavoie. (2017). Processing of the Amyloid Precursor Protein by the type II transmembrane serine protease matriptase. The Journal of Biological Chemistry 292 (50), 20669-20682. (Published).
  • Katsiaryna V. Gris*, Jean-Philippe Coutu*, Denis Gris. (2017). Supervised and Unsupervised Learning Technology in the Study of Rodent Behavior. Frontiers of Behavioral Neuroscience 11 (11), 141. (Published).
  • Zerif E, Maalem A, Gaudreau S, Guindi C, Ramzan M, Véroneau S, Gris D, Stankova J, Rola-Pleszczynski M, Mourad W, Dupuis G, Amrani A. (2016). Constitutively active Stat5b signaling confers tolerogenic functions to dendritic cells of NOD mice and halts diabetes progression. Autoimmunity 16 178-89. (Published).
  • L Takser, D Gris. (2016). Cyanotoxins at low doses induce apoptosis and inflammatory effects in murine brain cells: potential implications for neurodegenerative diseases. Toxicology reports 10 234-41. (Published).
  • Desbiens L, Lapointe C, Gharagozloo M*, Mahmoud S*, Pejler G, Gris D, D'Orléans-Juste P. Last two authors equally contributed to the publication. (2016). Significant Contribution of Mouse Mast Cell Protease 4 in Early Phases of Experimental Autoimmune Encephalomyelitis. Mediators of Inflammation 16 56-63. (Published).
  • Gharagozloo M*, Mahvelati TM*, Imbeault E*, Gris P, Zerif E, Bobbala D, Ilangumaran S, Amrani A, Gris D. (2015). The nod-like receptor, Nlrp12, plays an anti-inflammatory role in experimental autoimmune encephalomyelitis. J. Neuroinflammation 12 (1), 198-208. (Published).
  • Emilie Imbeault*, Tara Mahvelati*, Pavel Gris, Ralf Braun, Denis Gris. (2014). Nlrx1 regulates neuronal cell death. Molecular Brain 7 (1), 7-14. (Published).
  • Eitas TK , Chou WC , Wen H , Gris D , Robbins GR , Brickey J , Oyama Y , Ting JP. (2014). The nucleotide-binding leucine-rich repeat (NLR) family member NLRX1 mediates protection against experimental autoimmune encephalomyelitis and represses macrophage/microglia-induced inflammation. The Journal of Biological Chemistry 289 (7), 4173-9. (Published).
  • Imbeault E*, Gris D. (2013). Assessment of oxidative metabolism. Methods in Molecular Biology (Clifton, N.J.) 1031 1031:51-7. (Published).
  • Savard A , Lavoie K , Brochu ME , Grbic D , Lepage M , Gris D , Sebire G. (2013). Involvement of neuronal IL-1beta in acquired brain lesions in a rat model of neonatal encephalopathy. Journal of Neuroinflammation 10 (1), 10:110. (Published).
  • Wen H , Gris D , Lei Y , Jha S , Zhang L , Huang MT , Brickey WJ , Ting JP. (2011). Fatty acid-induced NLRP3-ASC inflammasome activation interferes with insulin signaling. Nature Immunology 12 (5), 408-15. (Published).
  • Allen IC, Moore CB, Schneider M, Lei Y, Davis BK, Scull MA, Gris D, Roney KE, Zimmermann AG, Bowzard JB, Ranjan P, Monroe KM, Pickles RJ, Sambhara S, Ting JP. (2011). NLRX1 protein attenuates inflammatory responses to infection by interfering with the RIG-I-MAVS and TRAF6-NF-?B signaling pathways. Immunity 34 (6), 854-65. (Published).
  • Hakimi M , Selvanantham T , Swinton E , Padmore RF , Tong Y , Kabbach G , Venderova K , Girardin SE , Bulman DE , Scherzer CR , LaVoie MJ , Gris D , Park DS , Angel JB , Shen J , Philpott DJ , Schlossmacher MG. (2011). Parkinson's disease-linked LRRK2 is expressed in circulating and tissue immune cells and upregulated following recognition of microbial structures. Journal of neural transmission (Vienna, Austria : 1996) 118 (5), 795-808. (Published).
  • Taxman DJ , Holley-Guthrie EA , Huang MT , Moore CB , Bergstralh DT , Allen IC , Lei Y , Gris D , Ting JP. (2011). The NLR adaptor ASC/PYCARD regulates DUSP10, mitogen-activated protein kinase (MAPK), and chemokine induction independent of the inflammasome. The Journal of Biological Chemistry 286 (22), 19605-16. (Published).
  • Arthur JC , Lich JD , Ye Z , Allen IC , Gris D , Wilson JE , Schneider M , Roney KE , O'Connor BP , Moore CB , Morrison A , Sutterwala FS , Bertin J , Koller BH , Liu Z , Ting JP. (2010). Cutting edge: NLRP12 controls dendritic and myeloid cell migration to affect contact hypersensitivity. Journal of immunology (Baltimore, Md. : 1950) 185 (8), 4515-9. (Published).
  • Huang MT , Mortensen BL , Taxman DJ , Craven RR , Taft-Benz S , Kijek TM , Fuller JR , Davis BK , Allen IC , Brickey WJ , Gris D , Wen H , Kawula TH , Ting JP. (2010). Deletion of ripA alleviates suppression of the inflammasome and MAPK by Francisella tularensis. Journal of immunology (Baltimore, Md. : 1950) 185 (9), 5476-85. (Published).
  • Gris D , Ye Z , Iocca HA , Wen H , Craven RR , Gris P , Huang M , Schneider M , Miller SD , Ting JP. (2010). NLRP3 plays a critical role in the development of experimental autoimmune encephalomyelitis by mediating Th1 and Th17 responses. Journal of immunology (Baltimore, Md. : 1950) 185 (2), 974-81. (Published).
  • Jha S , Srivastava SY , Brickey WJ , Iocca H , Toews A , Morrison JP , Chen VS , Gris D , Matsushima GK , Ting JP. (2010). The inflammasome sensor, NLRP3, regulates CNS inflammation and demyelination via caspase-1 and interleukin-18. The Journal of neuroscience : the official journal of the Society for Neuroscience 30 (47), 15811-20. (Published).
  • Desbiens L, Lapointe C, Gendron L, Gharagozloo M, Vincent L, Pejler G, Gris D, D'Orléans-Juste P. ExperimentalAutoimmune Encephalomyelitis Potentiates Mouse Mast Cell Protease 4-DependentPressor Responses to Centrally or Systemically Administered Big Endothelin-1. Pharmacol Exp Ther 370 (3), 446-457. (Published).

Chapitres de livre

  • Emilie Embault*, Denis Gris. (2013). Assessment of oxidative activity. Allen Coy. Mouse models of innate immunity (1st, 1031, 51-57). United States : Humana Press. (Published).

Articles de conférence

  • K Yamomoto*, K Gris*, S Mahmoud*, M Gharagozloo*, C Simard*, D Houle*, D Gris. (2017). Automated Long-Term Home Cage Behavioral Assessment as a Sensitive Tool to Assess Sex and Neuroinflammatory Differences in Mice. Canadian Association for Neurosciences. (Published).
  • D Gris. (2017). Computerized behavioral assessment of novel model of multiple sclerosis. Neuroimmunology and therapeutics. (Published).
  • D Gris. (2017). Endogenous negative regulators of inflammation preserve neuronal survival. Toxicology-pharmacology. (Published).
  • M Garhagozloo*, S Mahmoud*, K Yamomoto*, D Gris. (2017). Novel spontaneous model of MS. Canadian Association for Neuroscience. (Published).
  • C Simard*, S Mahmoud*, D Gris. (2017). Role of Nlrx1 in survival of astrocytes. XXIII Immunocoloque. (Published).
  • M Gharagozloo*, S Mahmoud*, C Simard*, K Yamamoto*, D Gris. (2016). NLRs as Endogenous Inhibitors of Inflammation in Multiple Sclerosis. EndMS Conference. (Published).
  • Marjan Gharagozloo, Denis Gris. (2016). Role of Nlrp12 in MS. Miami Immunology conference. (Published).
  • K Gris*, K Yamamoto*, S Mahmoud*, M Gharagozloo*, D Gris. (2016). Using Behavioral Profile as a Tool to Predict Neuroinflammation. CAN. (Published).
  • K Gris*, K Yamamoto*, M Gharagozloo*, S Mahmoud*, D Gris. (2016). Using Behavioral Profile to Forecast Neuroinflammation. EndMS Conference. (Published).
  • Marjan Gharagozloo, Denis Gris. (2015). Role of Nlrp12 in T cell activation. CAN. (Published).
  • Mahvelaty T, Imbeault E, Gris D. (2014). Nlrp12 inhibits inflammation during EAE. Canadian association of Neuroscience meeting. (Published).
  • Gris D. (2014). Role of NLRs in multiple sclerosis. 3rd international symposium in Neurology and therapeutics. (Published).
  • Mahvelaty T, Imbeault E, Gris D. (2014). Role of Nlrp12 in multiple sclerosis. SFN conference. (Published).
  • Imbeault E, Mahvelaty T, Gris D. (2014). Role of Nlrx1 in neuronal death. SFN conference. (Published).
  • Emmanuelle Imbeault, Dominique Pouline, Denis Gris. (2013). Expression levels of NLRX1 and Beclin-1 decrease following hypoxia and starvation treatments in N2a cells line. Canadian association for neuroscience conference. (Accepted).
  • Imbeault E, Mahvelaty T, Gris D. (2013). NLRX1 in neuronal death. Canadian association of Neuroscience meeting. (Published).
  • Mahvelaty T, Imbeault E, Gris D. (2013). Nlrp12 improves the course of EAE. Multiple Sclerosis Society of Canada conference. (Published).
  • Imbeault E, Mahvelaty T, Gris D. (2013). Nlrx1 promotes neuronal survival. Multiple Sclerosis Society of Canada conference. (Published).
  • Gris D. (2013). Role of Nlrs in multiple sclerosis. MS society conference. (Published).
  • Rahmani S, Gris D. (2013). Role of Nlrx1 in multiple sclerosis. SCI meeting. (Published).
  • D. Gris, Z. Ye, H. Wen, P. Gris, S.D. Miller, and J.P. Ting. (2010). Nlrp3 inflammasome mediates Th1 and Th17 responses during development of experimental allergic encephalitis. Society for Neuroscience. (Published).
  • M. Gharagozloo*, Simard C*, Yamamoto K*, Mahmoud SH*, Gris K*, El Baze J, Sandoval KC, Amrani A, Gris D. NLRX1 Prevents the Development of Spontaneous Experimental AutoimmuneEncephalomyelitis in Mice. ACTRIMS Forum.

Autres contributions

Cours enseignés

  • Topics in Immunology. IML720. (2014-02-19 à 2014-04-17).
  • Clinical immunology (neuroimmunology).

Activités de collaboration internationale

  • Collaborator. United States. Together with Dr. Pablo Devicari, we are dissecting the effect of inflammasome on aging. I am evaluating the behavioral profile of ASC knock-out aging mice.
  • Collaborator. Germany. In collaboration with Dr. Rosenstiel, we are looking at the role of Nlrx1 in astrocytes of CreLoxP mouse model.
  • Collaborator. United States. In collaboration with Dr. Stephen Miller, we are looking at the mechanism of development and progression of multiple sclerosis.
  • Collaborator. Germany. This collaborative project is funded by the government of Quebec International Collaboration Program, working together with Dr. Braun from the University of Bayreuth, we are looking at the mechanisms of neuronal cell death in yeast and mice.

Présentations

  • (2018). Behavioral Signature of Early Neuroinflammation: Small Changes – Big Impact. Dementia and neurodegeneration. Toronto, Canada
  • (2018). Comprehensive multi-parametric behavioral analysis of neuroinflammation. Neurosummit and Neuroimmunology. Montreal, Canada
  • (2018). New computerized method to detect neuroinflammation. Neuroimmunology. Toronto, Canada
  • (2017). Computerized behavioral analysis of novel model of multiple sclerosis. Neuroimmunology and therapeutics. Dallas, United States
  • (2017). Cyanotoxins at low doses induce apoptosis and inflammation in murine cells. CTOW meeting. Milton, Canada
  • (2017). Endogenous negative regulators of inflammation preserve neuronal survival. Toxicology-pharmacology. Toronto, Canada
  • (2015). Endogenous pathways to limit inflammation in the CNS. Neuroimmunology conference. San Francisco, United States
  • (2015). Nlrs in neurodegeneration. Invited lecture. Bayreuth, Germany
  • (2015). Role of mitochondria in neuronal death. One mitochondrion, many diseases. Sherbrooke, Canada
  • (2014). Role of Nlrs in multiple sclerosis. Neurology and Theraputics. Sanfrancisco, United States
  • Gris P. (2013). Educational Guided Networks Helping Students Transition from Undergraduate to Graduate Programs. The Western Conference on Science Education. London, Canada
  • (2012). NlrX1 and Nlrp12 in neuroinflammation. Symposium in Inflammation. Montreal, Canada
  • (2012). Role of Nlrs in neurodegeneration. Cancordia University. Montreal, Canada